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Peptide Vial Calculator

Peptide vial calculator

Vial mass, water added and an amount in, concentration, volume, U-100 syringe units and amounts per vial out.

Lyophilized peptides arrive as a dry powder measured in milligrams. Once a liquid is added, every measurement is a volume, and the conversion from mass to volume depends on two numbers: what the vial holds and how much liquid went in. This calculator does that conversion for any peptide and shows the result on a U-100 syringe scale.

Peptide calculator

Vial contents
mg in the vial
Diluent added (bacteriostatic water)
mL
Amount to measure
mcg
Syringe (U-100)
Syringe reading10.0units= 0.100 mL
0102030405060708090100
Concentration
2.50 mg/mL
2,500 mcg/mL
Volume
0.100 mL
Amount
250 mcg
Amounts per vial
20
20 whole

Results are arithmetic from the three numbers entered. U-100 scale: 100 units = 1 mL. Presets are figures reported in published and community sources, offered as shortcuts, not recommendations. The vial label is the number to trust.

Calculators with presets for a specific peptide

Each page has the vial sizes that peptide is usually sold in, a reconstitution chart and, where enough calculations have been logged, the setups entered most often. Blend pages also split each amount into its components.

How the calculation works

There are three steps, and each one is a single division or multiplication:

  1. Concentration = vial mass (mg) / liquid added (mL). A 5 mg vial with 2 mL is 2.5 mg/mL.
  2. Volume = amount (mg) / concentration. 0.25 mg at 2.5 mg/mL is 0.1 mL.
  3. Syringe units = volume (mL) x 100 on a U-100 syringe. 0.1 mL is 10 units.

Amounts per vial is the vial mass divided by the amount: 5 mg / 0.25 mg = 20. Adding more water lowers the concentration and makes each amount a larger volume, which is easier to read on the scale but needs more room in the syringe. The syringe units page covers the scale itself in more detail.

Quick reference

U-100 readings for common vial sizes and water volumes. The amounts are examples, not suggestions.

5 mg vial

WaterConcentration100 mcg250 mcg500 mcg
1 mL5,000 mcg/mL2 u0.02 mL5 u0.05 mL10 u0.1 mL
2 mL2,500 mcg/mL4 u0.04 mL10 u0.1 mL20 u0.2 mL
2.5 mL2,000 mcg/mL5 u0.05 mL12.5 u0.125 mL25 u0.25 mL
3 mL1,667 mcg/mL6 u0.06 mL15 u0.15 mL30 u0.3 mL

10 mg vial

WaterConcentration250 mcg500 mcg1 mg
1 mL10 mg/mL2.5 u0.025 mL5 u0.05 mL10 u0.1 mL
2 mL5,000 mcg/mL5 u0.05 mL10 u0.1 mL20 u0.2 mL
3 mL3,333 mcg/mL7.5 u0.075 mL15 u0.15 mL30 u0.3 mL
5 mL2,000 mcg/mL12.5 u0.125 mL25 u0.25 mL50 u0.5 mL

Calculator questions

How is peptide concentration calculated after reconstitution?

Divide the mass in the vial by the volume of liquid added. A 5 mg vial with 2 mL of bacteriostatic water is 2.5 mg/mL, which is 2,500 mcg/mL. The water does not change the total mass in the vial, only how concentrated it is.

How many units is 250 mcg from a 5 mg vial with 2 mL of water?

At 2,500 mcg/mL, 250 mcg occupies 0.1 mL, which reads 10 units on a U-100 syringe. The vial holds 20 amounts of 250 mcg.

What is a unit on an insulin syringe?

On a U-100 syringe, 100 units equal 1 mL, so 1 unit is 0.01 mL. A unit measures volume only. How much peptide a unit holds depends entirely on the concentration in the vial.

Does syringe size change the number of units?

No. A 0.3 mL, 0.5 mL and 1 mL U-100 syringe all use the same scale; a smaller barrel just holds fewer units (30, 50 or 100). The calculator flags a result that is larger than the barrel selected.

How many amounts are in one vial?

Divide the vial mass by the amount. A 10 mg vial holds 40 amounts of 250 mcg regardless of how much water was added. The calculator shows the exact figure and the number of whole amounts.

Is mcg or mg used in the calculation?

Either. 1 mg is 1,000 mcg. The calculator converts the amount to mg internally, so entering 250 mcg or 0.25 mg gives the same result.

Setup data on the peptide pages covers 4,225 logged calculations. About the data.