TB-500 reconstitution calculator
Concentration, draw volume and U-100 units for TB-500 vials from 2 to 30 mg.
TB-500 is the name research vendors use for a synthetic peptide related to thymosin beta-4. Depending on the supplier it is described either as a short fragment of that 43-amino-acid protein or as the full sequence, so the vial label is the reference for what it holds. Vials run from 2 mg to 30 mg, and community-reported amounts range from about 500 mcg to 2.5 mg.
That spread makes the water volume matter. A 2.5 mg amount from a 5 mg vial with 2 mL is a full 1 mL syringe, while the same amount from a 10 mg vial with 2 mL is half of one. The calculator returns the concentration, volume, U-100 reading and amounts per vial, and flags any reading larger than the syringe selected.
TB-500 calculator
- Concentration
- 2.50 mg/mL
- 2,500 mcg/mL
- Volume
- 0.200 mL
- Amount
- 500 mcg
- Amounts per vial
- 10
- 10 whole
Results are arithmetic from the three numbers entered. U-100 scale: 100 units = 1 mL. Presets are figures reported in published and community sources, offered as shortcuts, not recommendations. The vial label is the number to trust.
Setups entered by researchers
Not enough TB-500 calculations have been logged yet to publish a breakdown. A peptide's setups appear here once it passes 100 calculations and a setup has been entered at least 30 times. How the setup data works.
TB-500 reconstitution chart
U-100 readings (u) and volumes for each vial size and water volume. Columns are amounts that appear in published and community sources, listed for comparison only.
2 mg vial
| Water | Concentration | 500 mcg | 750 mcg | 2 mg |
|---|---|---|---|---|
| 1 mL | 2,000 mcg/mL | 25 u0.25 mL | 37.5 u0.375 mL | 100 u1 mL |
| 2 mL | 1,000 mcg/mL | 50 u0.5 mL | 75 u0.75 mL | 200 u2 mLover 1 mL |
5 mg vial
| Water | Concentration | 500 mcg | 750 mcg | 2.5 mg |
|---|---|---|---|---|
| 1 mL | 5,000 mcg/mL | 10 u0.1 mL | 15 u0.15 mL | 50 u0.5 mL |
| 2 mL | 2,500 mcg/mL | 20 u0.2 mL | 30 u0.3 mL | 100 u1 mL |
| 2.5 mL | 2,000 mcg/mL | 25 u0.25 mL | 37.5 u0.375 mL | 125 u1.25 mLover 1 mL |
10 mg vial
| Water | Concentration | 500 mcg | 750 mcg | 2.5 mg |
|---|---|---|---|---|
| 2 mL | 5,000 mcg/mL | 10 u0.1 mL | 15 u0.15 mL | 50 u0.5 mL |
| 3 mL | 3,333 mcg/mL | 15 u0.15 mL | 22.5 u0.225 mL | 75 u0.75 mL |
| 4 mL | 2,500 mcg/mL | 20 u0.2 mL | 30 u0.3 mL | 100 u1 mL |
20 mg vial
| Water | Concentration | 500 mcg | 1 mg | 2.5 mg |
|---|---|---|---|---|
| 2 mL | 10 mg/mL | 5 u0.05 mL | 10 u0.1 mL | 25 u0.25 mL |
| 4 mL | 5,000 mcg/mL | 10 u0.1 mL | 20 u0.2 mL | 50 u0.5 mL |
30 mg vial
| Water | Concentration | 500 mcg | 1 mg | 2.5 mg |
|---|---|---|---|---|
| 3 mL | 10 mg/mL | 5 u0.05 mL | 10 u0.1 mL | 25 u0.25 mL |
| 6 mL | 5,000 mcg/mL | 10 u0.1 mL | 20 u0.2 mL | 50 u0.5 mL |
How the TB-500 numbers are worked out
Concentration is the vial mass divided by the water: 5 mg in 2 mL is 2.50 mg/mL, 10 mg in 2 mL is 5.00 mg/mL.
The volume for an amount is the amount divided by the concentration; a U-100 syringe reads 100 units per mL. 2.5 mg from a 5 mg vial at 2 mL is 100 units (1 mL); from a 10 mg vial at 2 mL it is 50 units (0.5 mL).
Milligram-scale amounts at low concentrations take up more than one syringe. Only a higher concentration, meaning less water or a larger vial with the same water, brings the volume per mg down.
Research summaries and vendor comparisons for TB-500 are covered in a TB-500 research overview.
TB-500 calculator questions
How many units is 2.5 mg of TB-500 from a 10 mg vial?
With 2 mL of water the vial is 5.00 mg/mL, so 2.5 mg is 50 units (0.5 mL). With 3 mL it is 75 units (0.75 mL), and with 4 mL 100 units (1 mL). The vial holds 4 amounts of 2.5 mg.
How many units is 500 mcg of TB-500 from a 5 mg vial?
With 2 mL, 20 units (0.2 mL). With 1 mL, 10 units (0.1 mL). The vial holds 10 amounts of 500 mcg.
Why does a 2 mg TB-500 amount not fit in one syringe?
From a 2 mg vial with 1 mL, 2 mg is 100 units (1 mL), exactly one 1 mL syringe. With 2 mL it is 200 units (2 mL), twice what a 1 mL syringe holds. A 2 mg vial contains only one 2 mg amount whatever the water.
How are 20 and 30 mg TB-500 vials calculated?
The same way as any size. 20 mg with 4 mL and 30 mg with 6 mL are both 5.00 mg/mL, the same as 10 mg with 2 mL, so 1 mg is 20 units (0.2 mL) in each case. The larger vials hold 20 and 30 amounts of 1 mg.
Is TB-500 the same as thymosin beta-4?
Not always. Some suppliers use TB-500 for a short synthetic fragment of thymosin beta-4 and list the full-length protein separately; others use the names interchangeably. The arithmetic here uses only the mass printed on the vial label.
What TB-500 amounts do community sources report?
Community sources describe two broad patterns: smaller amounts around 500 mcg on a frequent schedule, and larger amounts of 2 to 2.5 mg on a less frequent one. The presets cover both ranges to save typing; they are not a recommendation.